454-flx pyrosequencing  (Pyrosequencing Inc)

Journal: Genes

Article Title: A Comprehensive Review of Bioinformatics Tools for Genomic Biomarker Discovery Driving Precision Oncology

doi: 10.3390/genes15081036

Figure Lengend Snippet: Overview of sequencing technologies 2004–2022. Post-Sanger sequencing technologies, beginning with Roche 454 Pyrosequencing in 2004, Illumina HiSeq and MiSeq (2007), SOLiD (2008), PacBioSMRT (2009), DNBS: Helicos (2010), Ion Torrent (2011), in situ RNA sequencing (2013), ONT nanopore (2015), spatial transcriptomics (2016), GeoSeq (2017), Slide-Seq (2019), and Revio (2022), illustrate the continuous improvements contributing to the genomics. It explores the various NGS sequencing techniques that have expanded upon chain termination and polymerase chain reaction.

Article Snippet: For example, Roche/454 FLX Pyrosequencer was the first high-throughput screening platform used in 2004 [ , ].

Techniques: Sequencing, In Situ, RNA Sequencing Assay, Polymerase Chain Reaction

Journal: BMC Oral Health

Article Title: The role of oral microbiota in the development of oral mucositis in pediatric oncology patients treated with antineoplastic drugs: a systematic review

doi: 10.1186/s12903-024-03938-y

Figure Lengend Snippet: Methodology of included studies

Article Snippet: Ye 2013 [ ] , Bacterial samples analyzed using 454 FLX pyrosequencing with minor modifications. The PCR products were sequenced using a two-lane PicoTiterPlate on a Genome Sequencer FLX system. Denoised sequences were aligned and sorted into operational taxonomic units (OTUs). , Qualitative analysis Prevalence Quantitative analysis (Unifrac).

Techniques: Bacteria, Control, Chocolate, Modification, Staining, Biomarker Discovery